Journal: Molecular Medicine Reports

Article Title: Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model

doi: 10.3892/mmr.2016.5080

Figure Lengend Snippet: Enterovirus 71 isolation and the general status of mice following inoculation with viral isolates. (A) Cytopathogenic effect on MA104 cells (×100). (a) control cells; (b) cells infected with low pathogenic JN200803 strain; (c) cells infected with highly pathologic JN200804 strain. (B) Mice were inoculated with the two viruses. Mice a–g, JN200804-infected; mouse h, representative JN200803-infected mouse. (C) Survival curves following infection with JN200804 and JN200803 strains. (D) Electromicrography time limit and wave amplitude results of JN200804-infected and JN200803-infected mice. Data are presented as the mean ± standard error. * P<0.05 vs. control group. The results presented are representative of three experiments.

Article Snippet: EV71 was detected using mouse monoclonal anti-EV71 antibody (1:1,000; EMD Millipore, Billerica, MA, USA; cat. no. MAB979) followed by incubation with goat anti-mouse immunoglobulin G peroxidase-conjugated antibody (1:200; BIOSS, Beijing, China; cat. no. bs-0296G).

Techniques: Isolation, Control, Infection

Journal: Molecular Medicine Reports

Article Title: Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model

doi: 10.3892/mmr.2016.5080

Figure Lengend Snippet: Histological examination of tissues from enterovirus 71-infected mice using hematoxylin and eosin staining. (A and B) Cerebellum, (C and D) spinal midpiece, (E and F) lung, (G and H) liver, (I and J) renal cortex and (K and L) skeletal muscle of JN200804-infected (A,C,E,G,I and K) and JN200803-infected (B,D,F,H,J and L) mice. In JN200804-infected mice, compared with negative controls, (A) the number of Purkinje cells and granular cells of cerebellum were decreased, (C) nerve fibers in white matter of spinal middle piece were thick, swollen looking. (E) Pneumorrhagia was also observed under microscope. (G) Lymphocyte infiltration and acidophilic degeneration was observed in the liver. (I) In the renal cortex, the number of glomeruli was decreased and epithelial cells within the kidney tubules appeared cloudy and swollen. (K) Necrolysis and lymphocyte infiltration was observed in skeletal muscle cells necrolysis with lymphocyte infiltration There was no apparent histopathological changes to the JN200803-infected mice compared with negative controls. The results presented are representative of two experiments.

Article Snippet: EV71 was detected using mouse monoclonal anti-EV71 antibody (1:1,000; EMD Millipore, Billerica, MA, USA; cat. no. MAB979) followed by incubation with goat anti-mouse immunoglobulin G peroxidase-conjugated antibody (1:200; BIOSS, Beijing, China; cat. no. bs-0296G).

Techniques: Infection, Staining, Microscopy

Journal: Molecular Medicine Reports

Article Title: Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model

doi: 10.3892/mmr.2016.5080

Figure Lengend Snippet: Immunohistochemical analysis. Immunohistochemistry staining of brain tissue from (A) JN200804-infected and (B) JN200803-infected mice. Arrows indicate enterovirus 71 antigen-positive cells (brown staining). The results presented are representative of two experiments. Staining used was 3,3′-diaminobenzidine and counterstaining with hematoxylin. Magnification, ×400.

Article Snippet: EV71 was detected using mouse monoclonal anti-EV71 antibody (1:1,000; EMD Millipore, Billerica, MA, USA; cat. no. MAB979) followed by incubation with goat anti-mouse immunoglobulin G peroxidase-conjugated antibody (1:200; BIOSS, Beijing, China; cat. no. bs-0296G).

Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Infection

Journal: Molecular Medicine Reports

Article Title: Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model

doi: 10.3892/mmr.2016.5080

Figure Lengend Snippet: CD4 T cells expansion is increases in highly pathogenic EV71-infected mice. Mice (n=5) were inoculated with EV71 JN200803 and JN200804 strains (1×10 4 tissue culture infectious dose 50 , intraperitoneal injection), controls were injected with phosphate-buffered saline. At 0, 3, 7 and 12 days post-inoculation, spleens were isolated and homogenized. Splenocytes were stained with anti-CD4 and CD25 antibodies, and intra-cellularly stained with anti-Foxp3 antibodies. Flow cytometry and FlowJo software were used to analyze the CD4 T cells. (A) Kinetics of CD4 T cells expansion. A significant increase of CD4 T cell expansion was observed at 3 days post-inoculation with the highly pathogenic EV71 JN200804 strain. Representative flow cytometry analysis of CD4 T cell population in (B) uninfected mice, and mice 3 days post-inoculation with EV71 (C) JN200804 and (D) JN200803 strains. The results presented are representative of three experiments. Data are presented as the mean ± standard error. ** P<0.01 vs. JN200803. CD, cluster of differentiation; EV71, enterovirus 71; Foxp3, forkhead box P3.

Article Snippet: EV71 was detected using mouse monoclonal anti-EV71 antibody (1:1,000; EMD Millipore, Billerica, MA, USA; cat. no. MAB979) followed by incubation with goat anti-mouse immunoglobulin G peroxidase-conjugated antibody (1:200; BIOSS, Beijing, China; cat. no. bs-0296G).

Techniques: Infection, Injection, Saline, Isolation, Staining, Flow Cytometry, Software